According to a report from Al Jazeera's Investigative Unit, Peyton Manning's wife was supplied with human growth hormones when the quarterback was recovering from an injury in 2011.
"The Dark Side: Secrets of the Sports Dopers," a documentary set to air on Sunday, was shared in advance with The Huffington Post and includes recordings of a Texas-based pharmacist named Charlie Sly, who was assisting Manning with his recovery process at the Guyer Institute, an Indiana-based anti-aging clinic.
"All the time we would be sending Ashley Manning drugs," Sly says in the video. "Like growth hormone, all the time, everywhere, Florida. And it would never be under Peyton's name, it would always be under her name."
In the recordings, Sly also says Manning and his wife came to the clinic after its normal business hours for intravenous treatments.
Manning was a member of the Indianapolis Colts at the time, but joined the Denver Broncos for the 2012 season.
The NFL banned the use of HGH in 2011, however the league did not begin testing until 2014 and it is believed that no player has tested positive.
Manning responded in a personal statement released by the Broncos late Saturday night.
Personal statement from Peyton Manning released by the Broncos ... http://pic.twitter.com/O5pJuwzWGF
— @AlbertBreer
The credibility of Sly's assertions have been called into question, and Manning's agent told Al Jazeera that Manning "has never done what this person is suggesting."
"The treatment he received at the Guyer Institute was provided on the advice of his physician and with the knowledge of team doctors and trainers," he said.
"Any medical treatment received by Ashley is a private matter of hers, her doctor, and her family."
Green Bay Packers linebacker Clay Matthews, Ryan Howard of the Philadelphia Phillies, Ryan Zimmerman of the Washington Nationals, and Mike Tyson are among those whom Sly claims he provided a substance called Delta-2, which he said is undetectable.
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